Evaluation of Methods for the Extraction of DNA from Drinking Water Distribution System Biofilms

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概要

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• While drinking water biofilms have been characterized in various drinking water distribution systems (DWDS), little is known about the impact of different DNA extraction methods on the subsequent analysis of microbial communities in drinking water biofilms. Since different DNA extraction methods have been shown to affect the outcome of microbial community analysis in other environments, it is necessary to select a DNA extraction method prior to the application of molecular tools to characterize the complex microbial ecology of the DWDS. This study compared the quantity and quality of DNA yields from selected DWDS bacteria with different cell wall properties using five widely used DNA extraction methods. These were further selected and evaluated for their efficiency and reproducibility of DNA extraction from DWDS samples. Terminal restriction fragment length analysis and the 454 pyrosequencing technique were used to interpret the differences in microbial community structure and composition, respectively, from extracted DNA. Such assessments serve as a concrete step towards the determination of an optimal DNA extraction method for drinking water biofilms, which can then provide a reliable comparison of the meta-analysis results obtained in different laboratories.

• 日本微生物生態学会・日本土壌微生物学会の論文

著者

• Liu Wen-tso

  Department Of Civil Engineering National University Of Singapore

• Hwang Chiachi

  Department of Civil and Environmental Engineering, University of Illinois Urbana-Champaign

• Ling Fangqiong

  Department of Civil and Environmental Engineering, University of Illinois Urbana-Champaign

• Andersen Gary

  Ecology Department, Earth Sciences Division, Lawrence Berkeley National Laboratory

• LeChevallier Mark

  American Water

関連論文

• Role of Glycogen in Acetate Uptake and Polyhydroxyalkanoate Synthesis in Anaerobic-Aerobic Activated Sludge with a Minimized Polyphosphate Content
• DNA Microarray Technology in Microbial Ecology Studies : Principle, Applications and Current Limitations
• Evaluation of Methods for the Extraction of DNA from Drinking Water Distribution System Biofilms
• Evaluation of Methods for the Extraction of DNA from Drinking Water Distribution System Biofilms

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