グラム陰性桿菌の線毛のアミノ酸組成,免疫学的性状および分子量の比較

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The fimbriae of the genera Shigella, Escherichia, Klebsiella, Aeromonas, and Serratia were purified. Their purities were proved by electron microscopy, immunodiffusion, and SDS polyacrylamide gel electrophoresis. The purified fimbriae were used to determine the molecular weight of the fimbrial subunits, and the amino acid composition and immunological properties of the fimbriae.The mannose-sensitive (MS-type) fimbriae of Shigella flexneri, Escherichia coli, and Klebsiella pneumoniae had some similarities in amino acid composition, containing more alanine, aspartic acid, and threonine. In them, neutral and acidic amino acids were abundant, but the molar percentage of basic amino acids ranged from 3 to 5. The fimbriae of Aeromonas hydrophila contained more glycine, alanine, and glutamic acid than those of any other organism. In contrast, the mannose-resistant type fimbriae of Serratia marcescens contained more lysine, which is a basic amino acid, aspartic acid, and alanine than those of any other organism. The molar percentage of basic amino acids was about 24. These results indicated that there was a marked difference in the characters of the fimbriae between Serratia and any others species.The MS-type fimbriae of the 3 species also shared remarkable similarity in immunologicall properties, but those of the other 2 species differed a little from each other.The molecular weights of the subunits of the fimbriae of all the bacterial species studied were estimated to be about 17, 000 by SDS polyacrylamide gel electrophoresis.
日本細菌学会の論文

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堀内三吉
東京医科歯科大学

グラム陰性桿菌の線毛のアミノ酸組成,免疫学的性状および分子量の比較

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