腸炎ビブリオ抗原の血清学的考察-3-O群別試験
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1. Immunizing and reacting O antigen of Vibrio parahaemolyticus was prepared in the following manner: Organisms were grown on nutrient agar containing 3% sodium chloride and incubated at 37 C overnight. They were harvested and suspended to the final concentration of about 10mg/ml in 3% sodium chloride solution containing 5% glycerol. The cell suspension was heated at 120 C for one hour or longer and then centrifuged. After centrifugation, the resulting supernatant was discarded and the packed cells were then resuspended in 1% sodium chloride solution.2. By the cross-agglutination and agglutinin-absorption tests, it was proved that the major antigen of O group 12 was identical with that of O group 10, but that the minor antigens were not. Therefore, O group 12 proposed by Miwatani et al, should be excluded from the antigenic schema of Vibrio parahaemolyticus at present.3. A total of 166 cultures of Vibrio parahaemolyticus which had been isolated from human patients and sea fishes were studied with O grouping antisera prepared by the authors. From the results of this study, it was found that all the cultures could be typed serologically into eleven O groups.4. For the serological typing of Vibrio parahaemolyticus, the O grouping test should be used together with the K antigen test.5. The K antigen of Vibrio parahaemolyticus seemed to be of the same character as the A type antigen of Escherichi coli named by Kauffmann and Knipschildt.
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