ヒツジ血球免疫ニワトリのrosette-forming cellsに関する研究
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The present studies are concerned with the cytodynamics, morphology and immunological nature of rosette-forming cells (RFCs) appearing in the peripheral blood and spleen of chickens following the immunization against sheep erythrocytes (SRBC). All the experiments were performed by the immunocyto-adherence technique. The results obtained were described and discussed in relation to the possible functions of RFCs in immune response.1. There was no significant difference in the cytodynamics and cytological characteristics of RFCs between the peripheral blood and spleen. First, large blast-like cells surrounded by several layers of erythrocytes (RFC-type 1) appeared. They were followed by plasmocyte-like cells surrounded similarly by several layers of erythrocytes (RFC-type 2), and then by small lymphocytes surrounded by one or two layers of erythrocytes (RFC-type 3). RFCs of the last type remained in small numbers until one to two months later.2. No RFCs were observed in bursectomized birds lacking in humoral antibody-producting capacity. In contrast, RFCs appeared in thymectomized birds with no cell-mediated immunity, showing essentially the same cytodynamics and morphology as those of the normal bird. It was, therefore, confirmed that RFCs were originated from bursa-dependent immunocompetent cells.3. The rosette formation of RFC-types 1 and 2 was remarkably suppressed, whereas that of RFC-type 3 was not affected by such inhibitors of metabolism concerning energy production as sodium azide, fluoroacetate, and dinitrophenol. The addition of complement induced a striking decrease in number of RFC-types 1 and 2, and a slight decrease in number of RFC-type 3. These findings suggest that there may be a distinct difference in the mechanism of rosette formation and entity of antibody or antibodylike receptors between RFC-types 1 and 2 as a group and the other RFC-type.4. Only a few RFCs were detected from the peripheral lymphocytes and splenic lymphoid cells of unsensitized normal birds after the cells were treated with anti-SRBC chicken serum in vitro or in vivo. Accordingly, it was ascertained that the rosette formation was not due to cytophilic antibody adsorbed onto these cells.From the results mentioned above, it may be concluded that RFC-types 1 and 2 are actively antibody-producing and releasing cells, while RFC-type 3 is a primed cell not having reached the stage of releasing antibody, or a memory cell playing an essential role in the secondary response.
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