緑膿菌エキスを抗原とするタンニン酸処理血球凝集反応慢性中耳炎患者血清での予備観察

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The authors made an attempt to determine whether or not humoral antibodies were present in patients of chronic otitis media with otorrhea which was positive for the presence of Pseudomonas aeruginosa. Sera from thirty-five patients were examined by Boyden's method for the hemagglutination reaction of tanned sheep red cells coated with an aqueous extract of the bacterial cells. It was common that these sera showed a positive reaction at a high titer (1/40 to 1/640; or higher titers being reached in some cases) when tested against the bacterial antigen of homologous origin or against that from their own otorrhea. Whereas, heterologous matchings usually presented much lower titers. Sera from healthy persons had inevitably very low titers (≤1/20) commonly against different antigens.Then, the authors applied the present technique to sera from rabbits given intravenous injection with a heated suspension of the bacteria. Nine strains classified into different serological types by Homma's method were selected for the study designed. No appreciable antigenic overlapping was proved among these strains when examined by the cross absorption test and the cross inhibition test by the use of the present hemagglutination method.These findings suggested that antigen or antigens which seemed to be specific for individual strains of P. aeruginosa might be responsible for the present reaction.
日本細菌学会の論文