ブドウ球菌の病原性に関する研究 (II) : 培養濾液分画における菌体外酵素の活性

概要

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Studies were carried out on 3 staphylococcal strains with special reference to the α- and β-hemolysin, coagulase, lysozyme, and deoxyribonuclease activities in the fractions of their concentrated culture filtrates. The strains used were the Terashima, E-97 and E-46. The virulence and some biological characteristics of them were described in the previous report. Each strain was cultured in modified Chesbros medium, from which a filtrate was obtained at 72 hours of incubation at 37°C. Results are summarized as follows.1) The coagulase and lysozyme activities were reliable parameters of the virulence, but the production of hemolysin, both α- and β-hemolysin, was not.2) Coagulase activity was not observed in strain E-97 or E-46 by the ordinary method, but was shown markedly in concentrated culture filtrates of both strains.3) Deoxyribonuclease activity was distinct in strains Terashima and E-97, but not so marked in strain E-46, though strains E-97 and E-46 were almost equal in virulence.4) The coagulase activity was always seen in fraction I in all the cases, suggesting that the essential nature of the enzyme might be the same in all the strains. The other enzyme activities appeared in fraction I in some cases and fraction II in another, suggesting that the nature of these enzymes might be different from one strain to another.
日本細菌学会の論文