嫌気性培養法に関する研究-1-黄燐培養法について

概要

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The phosphorus jar method (PJM) was compared with the steel wool method (SWM), a new type of anaerobic culture method induced from Parker's iron wool method (1955).The results obtained are as follows.1) Almost the same results were obtained from the cultivation of Veillonella and many species of Clostridium by both methods.2) Lactobacillus, Bifidobacterium, and Bacteroids could grow predominantly at any time when SWM was used. Especially, Bacteroides cultured by SWM was numerically much superior to that by PJM.3) In the direct cultivation of rectal feces, SWM was predominant over PJM with regard to not only the number of colonies but also the number of strains obtained.4) Some of the colonies grown by PJM were small and opaque.5) However, when PJM with addition of 10% CO2 was used, good results near those obtained by SWM were brought about.6) PJM was very inconvenient because of the danger from poisonous gas and inflammability.