Reiterトレポネーマ細胞画分の電子伝達系,とくにチトクロームの存在について
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概要
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Cells of the Reiter treponeme were disrupted by sonication and fractionated by differential centrifugation. The cell fractions were tested for the electron transport system. The particulate fractions gave a reduced with Na2S2O4 minus oxidized difference spectrum consisting of peaks at 630, 562, 530 and 429mμ. The peaks at 630 and 562mμ seem to correspond to α-bands of a2-and b-types of cytochromes respectively and the peak at 530mμ may be characteristic of a β-band of the cytochrome b. The high peak at 429mμ may correspond to a Soret band of the cytochrome b. A marked trough with its maximum at 460mμ may be attributed to flavoprotein. Since only the Soret region at 429mμ can scarcely be seen in the supernatant following centrifugation at 104, 000×g, the cytochromes of the treponeme appear to be associated with the cytoplasmic membrane and the membranous structure as in bacterial cells. Higher activity of succinic dehydrogenase was localized in the particulate fractions than the supernatant obtained by centrifugation at 104, 000×g. NADH2 oxidase activity was found both particulate and supernatant fractions, but was generally higher in the supernatant fraction.
- 日本細菌学会の論文
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