Vibrioの血球凝集反応について
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概要
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Investigations on the improvements of the techniques of haemagglutination test for the differentiation of vibrios were made using strains of <I>V. cholerae</I>, <I>V. eltor</I>, NAG vibrio, <I>V. parahaernolyticus</I> and <I>V. alginolyticus</I>.<BR>As the suspending medium of the red blood cells, PBS (pH 7.6, for tissue culture) was most excellent. One drop of 3% red blood cell suspension in PBS at pH 7.6 was placed on the glass slide, and sufficient amount of test organism (about 0.5mg) was rubbed into the drop with the loop and mixed well; then, visible clumping took place almost immediately.<BR>As for the species of the red blood cells, guinea pigs were most preferable; chick, rabbit and horse were next in order; sheep and ox were not found suitable.<BR>Test organisms grown in the media in which acidification takes place after the bacterial growth should not be used for this test. <I>V. eltor</I> cells grown in such condition failed to cause positive clumping.<BR>Barua and Mukerjee (1965) stated that red blood cells of guinea pigs are inadequate for this test, because of the occasional positive clumping in strains of<I>V. cholerae.</I>However, according to the results of this study, visible clumpings were also observed with red blood cells of the other species such as horse, rabbit and chick. Continued observation of the same strains of <I>V. cholerae</I> revealed that in some straips of <I>V. cholerae</I> haemaggliutination test turned to positive during the course of subculture, indifferent to S-R variation.
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