R因子の伝達阻害に関する研究
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概要
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Since Koyama and Akiba noticed the inhibitory effect of sodium deoxycholate on the transfer of multiple drug resistance (R) factor from E. coli to Shigella, substances such as bile, bile salt, cholic acid and dodecyl sulfate as surface active agents and chloramphenicol, 8-azaguanine, gramicidin J and mitomycin C as inhibitors of biopolymer synthesis were described to inhibit the transfer of R factor. However, the works so far appeared were not so precautious in testing whether the used concentration was lethal or not on the fate of both donor and recipient.In this study, several inorganic salts and antibiotics were tested for their inhibitory effect on the transfer of R factor, by estimating the inhibitory concentration on the transfer together with bacteriostatic concentration on both donor and recipient. As a result, a series of zinc compounds such as ZnCl2, Zn(CH3COO)2 and ZnSO4 were found to inhibit the transfer of R factor at the concentration without affecting the growth of both donor and recipient.Among them, ZnSO4 was most powerfull, and the site of action of this substance was further analysed. Pretreatment of donor or recipient did not affect the transfer, and did not reveal any eliminating effect of R episomes. Whereas the presence of ZnSO4 in the culture in which specific pairings of donor and recipient are proceeding was a necessary requisite for obtaining the inhibition. In those inhibited cultures, whenever ZnSO4 was removed from the medium, instantaneous recovery of the transfer was observed. Further analysis on the action of ZnSO4 was made by pursuing the transfer after blendor treatment for the pairing, and it appeared that ZnSO4 inhibited the specific pairing of donor and recipient like Pucks finding in which Zn++ ion inhibited the irreversible binding of T1 phage to E. coli B. As a matter of fact, concentrations of Zn++ ion effective for the inhibition of these two biological systems were almost the same.
- 日本細菌学会の論文