好塩菌101株のリポ多糖類
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概要
- 論文の詳細を見る
An attempt to prepare lipopolysacchride from a halophilic bacterium No.101 was made by extracting the bacterial cells with hot phenol-water using the Westphal's method. Further purification was carried out by freezing the phenol extract at -20°C for 48 hours with successful removal of the nucleic acid moiety and some other inert contaminants.The purified preparation thus obtained showed high pyrogenic activity for rabbits (0.01μg per kg) and potent precipitation activity, its titer being 10μg/ml as measured on capillary precipitation test. On Ouchterlony's agar diffusion gave no more than one sharp line of precipitation against homologous bacterial cells antiserum, indicating an evidence for immunological homogeneity.Chemical analysis of the preparation was follows: N. 2.8%, P. 3.2%, amino-sugar 5.0%, reducing-sugar 16.0%, chloroform-methanol extractable material 57.3%.
- 日本細菌学会の論文