Rickettsia orientalisの組織培養に関する基礎的研究

概要

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In an attempt to isolate Rickettsia orientalis from rodents by means of tissue culture method, a study with a number of growing cell culture in liquid medium was performed to find out the optimal conditions for the support of the growth of R. orientalis from the liver and spleen of the mice previously infected with this infectious agent. The following results are obtained.<BR>1) When the suspension of the infected liver and spleen at a concentration of 10% is seeded onto the cells and incubated for 60 minutes at 37°C., it was observed that one-tenth of the particles of the seeded agents was absorved.<BR>2) Among the cell cultures used, a noticeable difference is not observed in the growth of R. orientalis, although a slightly lower LD50 titer is estimated in tissue cultures with the culture of DK cells.<BR>3) R. orientalis can be easily cultivated and isolated from the specimens from the trapped rodents under the conditions described above.<BR>4) For long period cultivation and for the study of the fine morphological structure of R. orientalis, tissue cultures with Descement cells seems to be the most applicable.
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