Purification and Properties of L-Gulono-1,4-lactone Oxidase from Grifola Frondosa

概要

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L-Gulono-1, 4-lactone oxidase activity was detected in G. frondosa; therefore its properties were studied after purification. A 766-fold purified preparation of the enzyme from fresh fruit bodies was obtained by means of a seven-step procedure, the overall yield being 14%. The purified enzyme gave a single band on polyacrylamide gel electrophoresis and its absorption spectrum exhibited the characteristic of a flavoenzyme. The enzyme pro-duced L-ascorbic acid and H2O2, with L-gulono-l, 4-lactone (GL) as the substrate and oxygen as the electron acceptor, and was optimally active at around pH 7.0 and 45°C. Its molecular mass was determined to be 250 kDa on gel filtration, while the dissociated enzyme exhibited a molecular mass of 69 kDa on SDS-polyacrylamide gel electrophoresis, but the true molec-ular weight is unknown because of the trypsin treatment in the purification process. The apparent Km value for GL was 24±1 mi. Its substrate specificity was extremely high and, assuming that for GL to be 100, the following results were obtained, D-mannono-, 25: D-glu-cono-, 4; L-idono-, 3; L-galactono-l, 4-lactone, 2; and 15 other lactones tested, 0. It is pre-sumed that this enzyme is similar to animal GL-oxidase, ascomycetes D-arabinonolactone oxidase, etc.
財団法人学会誌刊行センターの論文
2001-06-01