A MICROBIOLOGICAL ASSAY OF INOSITOL BY CUP-PLATE METHOD WITH KLOECKERA APICULATA

概要

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A cup-plate technique was proposed for the microbioassay of inositol in natural products with Kloeckera apiculata as test organism. The optimum conditions for the assay were preferred as follows. Assay medium: Atkins basal medium with minor modifications and 1% agar, l.5-mm plate thickness, pH 6.0. Standard solution of inositol: pH 6.0-6.5. Inoculum size of test organism: 0.1 optical density in final concentration. Incubation: 27°C, 20-24 hr. In this method, a reproducible, clear and sharply defined growth zone was steadily obtained and a linear response to pure inositol solution containing 10-400μg per ml was demonstrated with a probable error of 10% on statistical analysis. The inositol contents in some natural products obtained by the plate method were nearly equal to those determined with the turbidimetric one and satisfactory results were also recorded in the recovery experiments. This method is simple, accurate and available for the routine assay of inositol in biological materials of high potency.
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