QUANTITATIVE DETERMINATION OF TOCOPHEROLS BY HIGH-SPEED LIQUID CHROMATOGRAPHY

概要

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A high-speed liquid chromatographic method for the determination of α-, β-, γ- and δ-tocopherols using a spectrophotometer or a spectrofluorometer, is described. The best results were obtained using a 2.3 mm i.d.×500mm normal phase partition column, JASCO-PACK WC-03-500 and a spectrofluorometer as a specific detector. A mixture of diisopropyl ether and n-hexane (2:98) was used as the mobile phase. A flow rate was set at 0.8ml/min. A mixture of pure α-, β-, γ- and δ-tocopherols or natural tocopherols in vegetable oils were separately determined by this method.
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