Studies on ascorbic acid metabolism in phenylhydrazine-treated rats.

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Phenylhydrazine administered to rats at a dose of 4 mg/100g body weight per day for 3 days, diminished the total ascorbic acid level in liver, kidney and spleen tissues. The total ascorbic acid level in blood was, however, elevated after phenylhydrazine treatment. The other effects of phenylhydrazine treatment were a reduction in the activities of Dglucuronoreductase, L-gulonooxidase, dehydroascorbatase and uronolactonase in liver. The dehydroascorbatase activity in kidney was also diminished after phenylhydrazine treatment. The changes in the activities of liver enzymes were found to be accompanied by a diminution in the level of reduced ascorbic acid and an elevation in the dehydroascorbic acid level. It was concluded that the biosynthesis of L-ascorbic acid from both Dglucuronolactone and L-gulonolactone as substrates, was reduced in phenylhydrazine-treated rats. The degradation of L-ascorbic acid by both liver and kidney was diminished by the phenylhydrazine treatment. It was further concluded that phenylhydrazine-treated rats tried to conserve the level of substrate for the synthesis of L-ascorbic acid, by reducing the hydrolytic cleavage of D-glucuronolactone. The elevated blood ascorbic acid level under the same condition was ascribed to the diminished catabolism of L-ascorbic acid.
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