Inhibitory Effect of Zn2+ on Rabbit Muscle Pyruvate Kinase and Reactivation by Histidine
スポンサーリンク
概要
- 論文の詳細を見る
Histidine was found to be an activator of rabbit muscle pyruvate kinase activity with a K1/2 value of 0.6mM. Carnosine and anserine are also effective, but only at much higher concentrations. Hyperbolic kinetics with phosphoenolpyruvate of the enzyme were found in either the presence or absence of histidine.Of a number of divalent cations tested, only Zn2+ was found to be an effective inhibitor of enzyme activity at low concentrations. The kinetic data suggested that Zn2+ acted as inhibitor as well as activator for the enzyme activity; a high affinity binding site was associated with Ki of approximately 4.8μM Zn2+ and a catalytic site was associated with Km of approximately 80μM Zn2+. Zn2+, which is associated to a high affinity binding site of the enzyme, was removed by the addition of histidine with a K1/2 of approximately 0.6mM. From these findings, histidine including anserine and carnosine in muscle may act as a chelating agent for the enzyme activity.
- 財団法人 学会誌刊行センターの論文
著者
-
玉木 七八
Laboratory of Nutritional Chemistry, Faculty of Nutrition, Kobe-Gakuin University
-
池田 建比古
Laboratory of Nutritional Chemistry, Faculty of Nutrition, Kobe-Gakuin University
-
木村 恵子
Laboratory of Nutritional Chemistry, Faculty of Nutrition, Kobe-Gakuin University
-
森岡 幸子
Laboratory of Nutritional Chemistry, Faculty of Nutrition, Kobe-Gakuin University
-
木村 恵子
Laboratory of Nutritional Chemistry, Faculty of Nutrition
-
池田 建比古
Laboratory of Nutritional Chemistry, Faculty of Nutrition
-
玉木 七八
Laboratory of Nutritional Chemistry, Faculty of Nutrition
-
森岡 幸子
Laboratory of Nutritional Chemistry, Faculty of Nutrition
関連論文
- EFFECT OF HISTIDINE-FREE AND -EXCESS DIETS ON ANSERINE AND CARNOSINE CONTENTS IN RAT GASTROCNEMIUS MUSCLE
- EFFECT OF DIETARY METHIONINE LEVEL ON ANSER INE AND CARNOSINE CONTENTS IN THE GASTROCNEMIUS MUSCLE OF RAT
- The In Vivo Decrease of β-Alanine-2-Oxoglutarate Aminotransferase Activity Caused by-6-Azauracil and 5-Fluorouracil
- Inhibitory Effect of Zn2+ on Rabbit Muscle Pyruvate Kinase and Reactivation by Histidine
- INHIBITORY EFFECTS OF Zn2+ ON MUSCLE GLYCOLYSIS AND THEIR REVERSAL BY HISTIDINE
- BIOSYNTHESIS AND DEGRADATION OF CARNOSINE AND TURNOVER RATE OF ITS CONSTITUENT AMINO ACIDS IN RATS
- METABOLISM OF Nπ -METHYLHISTIDINE IN RATS
- ANSERINE AND CARNOSINE CONTENTS IN MUSCULAR TISSUE OF RAT AND RABBIT
- The utilization of carnosine in rats fed on a histidine-free diet and its effect on the levels of tissue histidine and carnosine.
- Effect of pyridoxine deficiency and prednisolone on .BETA.-alanine-oxoglutarate aminotransferase and D-3-aminoisobutyrate aminotransferase in rat liver and kidney.
- Inhibitory effect of 6-azauracil on .BETA.-alanine metabolism in rat.
- Submitochondrial localization of rat liver .BETA.-alanine-oxoglutarate aminotransferase.
- Carnosine as a histidine source: Transport and hydrolysis of exogeneous carnosine by rat intestine.