High-performance liquid chromatographic method for the analysis of N.TAU.-methylhistidine in food, chicken excreta, and rat urine.

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概要

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• A reliable high-performance liquid chromatographic method that utilizes a reversed-phase separation with ion-pairing and postcolumn fluorescence derivatization for the analysis of Nτ-methylhistidine in food, chicken excreta, and rat urine is described. Nτ-Methylhistidine in the hydrolyzed sample is first roughly separated from acid and neutral amino acids by an ion-exchange column. The Nτ-methylhistidine fraction is then evaporated and the residue is dissolved in the mobile phase (15mM sodium octane sulfonate in 20mM potassium phosphate), and subjected to high-performance liquid chromatography.

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著者

• 冨田 裕一郎

  Department of Animal Science, Faculty of Agriculture, Kagoshima University

• 豊水 正昭

  Department of Animal Science, Faculty of Agriculture, Kagoshima University

• 林 國興

  Department of Animal Science, Faculty of Agriculture, Kagoshima University

• 前田 芳實

  Department of Animal Science, Faculty of Agriculture, Kagoshima University

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• High-performance liquid chromatographic method for the analysis of N.TAU.-methylhistidine in food, chicken excreta, and rat urine.

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