Change of prolyl hydroxylase activity during secondary palatal closure of rat.
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概要
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The study reports on the direct extraction of prolyl 4-hydroxylase and prolyl 3-hydroxylase from the secondary palate of rats and the developmental changes in its activity over the time course of palatal closure. Pregnant female Wistar rats were killed with dimethyl-ethel, and fetuses were rapidly taken from the 14.5 th day to the 20 th day uterus.<BR>The following results were obtained;<BR>1) There was a strong relationship between embryonic day and embryonic weight.<BR>2) The activity of prolyl 3-hydroxylase per extracted protein could be measured on the 19 th day and 20 th day.<BR>3) The character of prolyl 4-hydroxylase of the rat was almost the same as the ones in the previous report.[Hutton et al. 1966.]<BR>4) The activity of prolyl 4-hydroxylase per extracted protein was high from the 16 th day to the 17 th day, just at the time of palatal shelf on the stage of fusion.<BR>The activity in the palatal shelves were decreased between days 17 th and 18 th.<BR>And from the 19 th day to the 20 th day, the activity increased again.
- 社団法人 日本口腔外科学会の論文
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- Change of prolyl hydroxylase activity during secondary palatal closure of rat.