A study of nuclear DNA content as prognostic value of squamous cell carcinoma in the oral and maxillofacial region. A retrospective study on relation of nuclear DNA content to clinical and histological factors.:A retrospective study on relation of nuclear
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230 cases of primary squamous cell carcinoma of the oral and maxillofacial region were observed to determine the content of nuclear DNA by Feulgen-DNA microspectrophotometry at Department of Oral and Maxillofacial Surgry, Kobe University Hospital. The author reports here:<BR>1) A new cell isolation technique for paraffin embedded tissue of squamous cell carcinoma<BR>2) Relation between nuclear DNA content and clinical, histological characteristics<BR>3) Prognostic factors by multiple regression analysis<BR>There are various techniques reported in the field of microspectrophotometry for cell isolation from paraffin embedded tissue, but none of which was found to be successful for squamous cell carcinoma. The author has, therefore, established a new cell isolation technique suitable for paraffin embedded tissue of squamous cell carcinoma. The most unique feature is to make it easy to pick up a small tissue direct from a thick paraffin specimen, and collect a large number of cells. The nuclear DNA content was analyzed in comparison with clinical, histological characteristics and statistical observation. The data on the nuclear DNA content was evaluated by histogram, classified into the following items;(1) Mode, (2) Frequency of Mode, (3) Number of cells greater than 4n, (4) Range, (5) Histogram Pattern, (6) Mean. Results indicate:<BR>-Most cases of aneuploidy cell division pattern showed poor prognosis.<BR>-Most cases of previous surgical treatment (tooth extraction, incision, puncture, etc.) at othr medical and dental units prior to this department showed polyploidy nuclear DNA content, aneuploidy cell division, and poor prognosis.<BR>-As a result of multiple regression analysis, it was found that on factors of previous surgical treatment at other medical and dental units and aneuploidy pattern of histogram adversely affected prognosis.<BR>Amongst histogram evaluation items, histogram pattern in representative of malignancy of squamous cell carcinoma.<BR>These facts indicate that nuclear DNA content of squamous cell carcinoma reflects a biological property to represent the malignancy of tumor and has significant relation to clinical and histological characteristics.<BR>And these findings suggest that the prognostic factors are of most importance for prospcctivc therapy of squamous cell carcinoma of the oral and maxillofacial region.
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