Cell attachment to the surface of silicon-apatite composite.
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概要
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In order to improve the physical property and the handling of hydroxyapatite ceramics, we devised and produced an entity of silicon-apatite composite. Using a scanning electron microscope, we observed the shape of the surface of this composite and evaluated the cell attachment to the surface by using culture cells.<BR>By scraping the surface of the composite, we could observe the exposed apatite particle that was coated with silicon. Although HeLa S<SUB>3</SUB> cells fully failed to attach to the surface of the silicon favorable cell attachment of these cells to the surface of the apatite could be observed. On the other hand, the cells presenting themselves in round shape were somewhat firmly attached to surface of the composite. A comparison of the cell attachment by means of colony formation on surface of the composite as an index revealed that the increased number of colonies formed on the surface was proportional to the increased ratio of apatite content. In the composite containing 70% apatite, the number of colonies was about 91% of the number of the disseminated cells, showing a favorable cell attachment to this composite.
著者
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柳谷 謙一
東京大学医学部口腔外科学教室
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水谷 雄
東京医科歯科大学歯学部第1口腔外科学教室
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横矢 重俊
東京医科歯科大学歯学部第1口腔外科学教室
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赤川 徹弥
東京医科歯科大学歯学部第1口腔外科学教室
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