Cellular kinetics and lectin distribution in DMBA-induced buccal skin carcinoma in rats.
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概要
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Rat buccal skin, to which a 9, 10-dimethy1-1, 2-benzanthracene acetone solution was applied, has been examined by various immunohistochemical techniques in order to elucidate a tumoral malignancy by examining the cellular kinetics and the lectin binding. The cellular kinetics were evaluated by identifying the DNA synthetic cells (S phase cells) by using 5-bromodeoxyuridine (BrdU) and the binding by the cells to 4 different lectins, such as the PNA, WGA, UEA-1 and RCA-1 during the process of carcinogenesis.<BR>The labeling index (LI) for BrdU amounted to 4.1% in normal epithelium, 15.2% in hyperplastic epithelium, and 17.8% in atypical epithelium. The higher the epithelial dysplasia degree, the higher the LI. Reflecting this, the LI was found to be 25.0% in a well differentiated squamous cell carcinoma (SCC) and 25.5% in a poorly differentiated carcinoma. There was no correlation, however, between the degree of the cell differentiation and the LI. BrdU-positive cells were found localized in the basal cell layer in both normal and hyperplastic epithelium, whereas they were distributed from the basal layer to the prickle cell layer in atypical epithelium.<BR>It was found that the binding of the tumor cells to the PNA, WGA and UEA-1 differed during the process of carcinogenesis. PNA and UEA-1, for instance, were negative in areas where BrdU-positive cells were present in normal and in hyperplastic epithelium, but were strongly positive in the cells of the lower portion of the prickle cell layer and in some areas of the basal cell layer in SCC. PNA also was positive in some BrdU-positive cells.<BR>These results indicate that evaluating the cell kinetics by using this BrdU technique and investigating the lectin binding may serve as a useful parameter in gauging a tumoral malignancy and reaching a diagnosis.
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