Electron microscopic detection of Epstein-Barr virus in oral hairy leukoplakia. Application of negative staining with Airfuge.:Application of negative staining with Airfuge<SUP>®</SUP>

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Oral hairy leukoplakia (HL) is a newly recognized virus-associated lesion of the oral mucosa that occurs in persons infected with human immunodeficiency virus (HIV). Recent studies have demonstrated Epstein-Barr virus in the epithelial cells of HL.<BR>The authors examined 69 HIV-positive patients and 4 HIV-negative patients by a negative staining method using a transmission electron microscopy (TEM). For negative staining, swab specimens were taken from the lateral border of the tongue in each patient. 146 specimens were examined with TEM after Airfuge ultracentrifugation, and 24 specimens were examined without Airfuge ultracentrifugation.<BR>Ultracentrifugation with Airfuge produced an approximately 3-fold increase in the detection of Epstein-Barr virus particles by TEM. Negative staining with Airfuge ultracentrifugation has advantages of simplicity and speediness for the clinical diagnosis of HL.
社団法人日本口腔外科学会の論文