Experimental studies of osteoinduction by a bone-inducing substance extracted from bovine bone matrix.
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概要
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inducing substance (cortical bone factor: CBF) extracted from bovine bone matrix was implanted into the femoral muscle of a mouse to make morphological observation of the osteogenetic induction process.The following results were obtained;<BR>1. Three days after CBF implantation, fibroblasts were proliferated around implanted CBF, seven days after the implantation undifferentiated mesenchymal cells were accumulated, and ten days thereafter the cartilage was formed.Fourteen days after the implantation resorption of the cartilage and addition of the new bone began with fibrous tissue and blood vessels being penetrated therein.Twenty one days post operatively, uneven bone was formed and bone marrow with sinusoidal capillaries was seen.This indicated the process of endochondral ossification. Twenty eight days after the implantaion, taking the form of the medulla and round cortical bone surrounding the medulla just as the long bone was cut crosswise, bone marrow gradully became so-called lipomedullary, but thirty five days thereafter, no great change was noticed.<BR>2. Three days after CBF implantation, myofibrous interfascicular connective tissue of the thigh showed <SUP>3</SUP>H-thymidine-marked cell rate of 11.7±1.4%for fibroblasts compared with 28.3±0.8% for those around the implanted CBF.<BR>3. <SUP>35</SUP>S-microautoradiogram seven days after CBF implantation revealed undifferentiated mesenchymal cells accumulated in the site of CBF implantation and the most grains accumulated thereabout;ten days after the implantation, chondrocytes and surrounding substrates showed grain accumulation, but not fourteen days thereafter.<BR>4. von Kossa staining indicated that calcification began with perichondro-matrix, then the added new bone.<BR>The above results revealed that CBF caused induction of the cartilage, followed by osteogenesis with endochondral ossification.
- 社団法人 日本口腔外科学会の論文
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