Histochemical study on mucosubstances of human normal submandibular gland by lectin staining with chemical modification.
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概要
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Histochemical properties of mucosubstances derived from human normal submandibular glands of 20 autopsied cases were investigated by staining with 9 different kinds of lectins including ConA, UEA-I, GS-I, GS-II, BPA, DBA and WGA. Immunohistochemical staining with anti-lysozyme antibody was also carried out to identify serous cells. Furthermore, prior to lectin binding, oxidation with periodic acid followed by reduction with sodium borohydride was performed in order to compare the tissue-specific staining properties of simple lectin stain with those after chemical modification. GS-II exclusively reacted with mucous cells and staining was enhanced by oxidation. SBA and DBA lectins also bound remarkably to mucous cells after chemical modification. No lectins reacted only with serous cells, and only UEA-I showed obvious signs of reaction with serous cells even after 10-min oxidation, while the other lectins exhibited minimal binding. DBA and BPA lectins were characterized by the existence of site-specific staining in intercalated duct cells. SBA showed strong binding with cells of the striated ducts and interlobular ducts. In addition, the existence of cells possessing mixed characteristics of serous and mucous cells was suggested in the acini, since glycoproteins and lysozymes were present in the same cytoplasm.<BR>These results indicated that cell-species of the human submandibular glands could be differentiated by staining techniques employing appropriate combinations of lectins and chemical modification. These techniques may be useful in studying the mechanisms of cell differentiation and tumorgenesis.
- 社団法人 日本口腔外科学会の論文