顎骨嚢胞組織から産生されるサイトカインに関する研究

概要

論文の詳細を見る
The mechanism of bone resorption in cystic lesions in bone tissue still remains to be elucidated. As various cytokines have recently been shown to be involved in bone resorption, the present study was undertaken to examine the relationship between cytokine production and bone resorption in cystic lesions.<BR>Tissues were obtained from 19 patients with histopathologically confirmed jaw cysts (7 with odontogenic keratocysts, 5 with radicular cysts, and 7 with dentigerous cysts). The cystic tissues were aseptically resected, cut into approximately 2-mm square pieces, and cultured for 48 hours in α-MEM containing 1% fetal bovine serum in 24-well plates. IL-6 and IL-1 in the supernatant were assayed by enzyme linked immunosorbent assay (ELISA). Northern blotting analysis was also carried out to study gene expression of the cytokines.<BR>The production of IL-6 was significantly greater in the radicular cysts than in the odontogenic keratocysts (p< 0.05). However, no correlation was found between the levels of IL-6 and IL-1, the latter of which is regarded to be an inducer of IL-6 production. Northern blotting analysis showed gene expression of IL-6 in cystic tissues, and TGF-β gene was expressed in the odontogenic keratocysts only. These results suggest that these cytokines are associated with the growth of jaw cysts.
社団法人日本口腔外科学会の論文