高分化型口腔扁平上皮癌におけるフィブロネクチン遺伝子の発現と予後に関する研究
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Biological studies on the extracellular matrix (ECM) and related cell receptors in cancer tissue have demonstrated that fibronectin (FN), which is one type of ECM, is involved in adhesion, cell activation and migration. However, the origin and the biological roles of FN which exists in cancer tissue and whether or not FN inhibits tumor proliferation are largely unclear.<BR>To elucidate the origin and the biological roles of FN in cancer tissue, the expression of FN was investigated by immunohistochemical staining and in situ hybridization technigues using an image analysis system for 80 well-differentiated oral squamous cell carcinomas (S. C. C) obtained by biopsy.<BR>The immunohistochemical distribution and the gene expression of FN were compared and evaluated in 80 cases of cancer tissue, and FN localization was discussed in regard to T classification, metastasis to regional lymph nodes (UICC 1987), and prognosis.<BR>1) FN distribution in well-differentiated S.C.0 was classified into 4types: negative, type N; FN positive in cancer parenchyma, type T; FN distributed in membrane-like fashion in cancer parenchyma, type M; diffuse distribution of FN in the cancer stroma, type D.<BR>2) Cases with numerous cells expressing FN m RNA in the stroma showed higher frequencies of weak and strong positive FN immunostaining than negative staining (p<0.001). There was no correlation between FN and FN m RNA expression in cancer parenchyma.<BR>3) Cases with numerous cells expressing FN m RNA in cancer parenchyma were significantly more frequent among type T and M than among type D (p<0.001). D-type tumors showed a higher frequency of cells expressing FN m RNA (p<0.001).<BR>4) T3 and T4 well-differentiated oral S. C. 0 were characterized by large numbers of cells expressing FN m RNA in cancer parenchyma and small numbers of cells expressing FN m RNA in stroma (p<0.001, p<0.05).<BR>5) Well-differentiated oral S. C. C. with regional lymph node metastasis frequently exhibited expression for FN m RNA in large numbers of cells in cancer parenchyma (p<0.001).<BR>6) Oral S. C. C. with good prognoses showed higher numbers of cells expressing FN m RNA in stroma and type D (p<0.05, p<0.025).
- 社団法人 日本口腔外科学会の論文