PCR-RFLP法によるネギアザミウマの2生殖型の識別と鳥取県におけるネギアザミウマ産雄性単為生殖型の分布
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To discriminate between arrhenotokous and thelytokous forms of the onion thrips (Thrips tabaci), we developed a simple molecular diagnostic method that uses PCR-RFLP of the mitochondrial cytochrome c oxidase subunit I (COI) gene. Digestion of 490-bp COI amplicons with EcoO109I provided reproductive form-specific RFLP patterns. Using this method, we surveyed the frequencies of the two reproductive forms in 9 Welsh onion fields in Tottori Prefecture. Arrhenotoky dominated in the western area, although 100% of individuals were thelytokous in the eastern area. The COI haplotype of the arrhenotokous individuals was haplotype 1 or 2, which has not previously been found in Japan.
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関連論文
- ナガイモにおけるコガネムシ類の生態と防除技術
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- PCR-RFLP法によるネギアザミウマの2生殖型の識別と鳥取県におけるネギアザミウマ産雄性単為生殖型の分布
- 鳥取県の砂丘畑においてナガイモを加害するタネバエの発生生態