蔗糖の歯質脱灰性に関する研究
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概要
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The purpose of this study was to examine the effect of sucrose upon the solubility of the dental hard tissues.<BR>Enamel and dentine powder prepared from freshly extracted bovine teeth was suspended in the following 3 solutions, namely pH 7.5 tris-buffer solution (B), mol sucrose in solution B (S) and solution S autoclaved at 125°C for 1hr (CS). After 16 days incubation at 37°C, the amounts of Ca and P dissolved in each solution were determined. Solution S increased the solubility of Ca and P in dentine, but decreased it in enamel in comparison with solution B. Solution CS significantly increased the solubility of Ca and P in both enamel and dentine.<BR>The free N-terminal groups in enamel and dentine incubated in solution B and solution CS for 16 days were determined by the use of Sanger's fluorodinitrobenzene method. No difference was found between the two solutions.<BR>In tooth powder deprived of its organic components by extraction with ethylendiamine, in natural apatite powder and in lipid-extracted teeth powder, the solubility tests were carried out under similar experimental conditions.<BR>The results indicated that the amounts of dissolved Ca and P were strongly influenced by the extraction of the lipid component.<BR>In the caramelized sucrose solution, lactate, formate, acetate and pyruvate were found. Any one of these acids was able to decrease calcium ion concentration in slightly alkalized CaCl<SUB>2</SUB> solution.
- 有限責任中間法人 日本口腔衛生学会の論文