解糖系酵素におよぼすフッ化物の影響(F Series-12-)enolase

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The effect of fluoride on purified enolase and the supernatant of mouse muscle homogenate, was studied in vitro and the change of enolase activity in mouse muscle was determined after feeding fluoride water of graded concentration for a week. The crude enolase in homogenate supernatant was more strongly inhibited than purified enolase, particularly when the activity was assayed by the sample mixed with fluoride in advance, namely as high as 50% inhibition in 0.5mM F. The author introduced a new hypothesis of incomplete inhibition (reversible) and complete inhibition (irreversible). In the latter case, it required the third factor, i. e. homogenate factor (H-factor), to be fully demonstrated. It was contained in the homogenate and probably comosed of phosphate, magnesium or other divalent metal and special protein. With the H-factor, the enolase became very sensitive to fluoride and was strongly and irreversibly inhibited. The inhibition went on to the greater extent with time.<BR>In vivo experiment, the slight inhibition of enolase activity was only observed at 100 and 400 ppm F feeding. The level was, however, so high that other enzyme systems might also be disturbed and the result might not be attributed solely to the effect of fluoride.
有限責任中間法人日本口腔衛生学会の論文