DL-2-ヒドロキシ-4-メチルチオ酪酸を前駆物質とするL-メチオニン生産の条件および機構の研究(2-ヒドロキシ酸よりL-アミノ酸の生成-2-)

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概要

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• In order to find out the proper conditions for L-methionine production from DL-2-hydroxy-4-methylthiobutyric acid (DL-HMBA) by fermentation with Pseudomonas denitrificans G-132-13, which was chosen in the previous paper as a suitable strain for the production, the effect of cultural conditions was studied. As a result of the studies, this strain gave 89% of theoretical yield of L-methionine, 20.8mg/ml, in 49 hours submerged culture with following medium: DL-HMBA ammonium salt, 2.35% (as free acid); glucose, 5%; corn steep liquor, 2%; ammonium chloride, 0.25%; pH, 7.8. The conversion of DL-HMBA to L-methionine was catalyzed with two enzyme systems, NAD, NADP-unlinked DL-HMBA dehydrogenase and transaminase.

• 社団法人 日本農芸化学会の論文

著者

• 和田 泱夫

  住友化学工業株式会社医薬事業研究部

関連論文

• DL-2-ヒドロキシ-4-メチルチオ酪酸を前駆物質とするL-メチオニン生産の条件および機構の研究(2-ヒドロキシ酸よりL-アミノ酸の生成-2-)

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