浮草の暗処理による低分子化合物含量の変化

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Short-day plant Lemna perpusilla was fed with 14CO2 under continuous illumination or short-day conditions. After almost all of 14CO2 was utilized, the duck weeds were extracted with 80% ethanol, and the ethanol extracts were developed with two dimensional paper chromatography. Eleven compounds on the autoradiogram of ethanol extracts have been identified. They were citric acid, malic acid, aspartic acid, glutamic acid, serine, asparagine, glutamine, sucrose, glucose, fructose and NAD. Among these compounds, citric acid, malic acid, aspartic acid, asparagine and glutamine were clearly changed in concentration at various conditions. Citric acid and aspartic acid were accumulated in short-day condition, while malic acid and glutamine were decreased in the same condition. The amount of asparagine showed an interesting changes as follows: when the photoperiod was changed from continuous illumination to short-day condition, asparagin was increased at the beginning of short-day condition and was decreased after flowering.
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