土壌細菌によるコメン酸,5-オキシマルトールの蓄積について

概要

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During the course of an investigation of the accumulation of maltol (3-hydroxy-2-methyl-γ-pyrone) by microorganisms, it was found that one strain No. 189, screened out from soil, accumulated two γ-pyrone compounds in the culture medium. The present investigation was undertaken to clarify the progress of fermentation with the strain and to identify the two γ-pyrone compounds. No. 189 strain was gram-negative and motile by means of polar flagella (lophotrichous). The strain produced water-soluble yellow pigment and 2-keto gluconic acid in the culture medium. On the basis of these data, it seemed reasonable to assume that No. 189 strain was belonging to Pseudomonas. The culture medium used contained 5% of sucrose, 1% of peptone, 0.1% of K2HPO4, 0.05% of MgSO4 and 0.1% of yeast extract. Incubation was carried out for 15 days at 37°C using 500-ml shaking flask. In this fermentation, as 2-ketogluconic acid was produced, the organism could not grow after 7 days, but the two γ-pyrone compounds were accumulated 855μg/ml as kojic acid after 15 days. In view of the fact that the two crystallized γ-pyrone compounds had identical IR absorption spectra and UV absorption spectra with authentic comenic acid and 5-oxymaltol, we concluded that one is comenic acid and the other is 5-oxymaltol.