分配クロマトグラフ法によるタンニン及び色素の研究 : (第2報)丸型濾紙クロマトグラフ法によるルチンの簡易定量法

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The principle of this method is based on the fact that when rutin is developed on the filter paper by n-butanol, its Rf value is smaller than that of other quercetin-3-glycoside pigments. One drop of rutin solution is spotted at the centre of round filter paper and it is developed circularly outwards by n-butanol saturated with water in the desiccator as shown in Fig. 1. After developing, the paper is dried and a yellow-coloured ring is obtained by spraying a basic lead acetate solution. From the width, i.e. the difference r1-r2, of this ring, its area is calculated as shown in Fig. 2. In the concentration range between 5 and 25mg of rutin in 10cc, there is a linear relation between the area of the ring and the logarithm of rutin content as shown in Fig. 3 and Table 2.
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