鹽類溶液を用いるPaper Chromatograpyと其應用
スポンサーリンク
概要
- 論文の詳細を見る
HORNE and POLLARD(1) reported a paper strip chromatography using 3% ammonium, chloride as a developing agent for identification of streptomycin. Allied methods were announced for separation of enzymes or proteins on the filter paper strips. We improved this method using 3% NaCl instead of ammonium chloride for chromatography of several antibiotics(2). We applied this method further for amino acids, pigments and vitamins. Formerly this phenomenon was considered as salting out, but recently ELTON(4) and RUTTER(5), declared it due to the streaming potential when the salt solution flows through the capillary channel of paper. 1) Rf values of natural amino acids by the salt solution: The procedure is almost the same as ordinary paper partition chromatography of amino acids with the use of NaCl as developer instead of organic solvents. (Table 1) It may be noted that the Rf values of most amino acids are about 0.9, whereas tyrosine, tryptophan and histidine have dferent Rf values. 2) Rf values of pigments: It is convenient that all pigments show spots directly of their own colors on the paper. The pH of the NaCl solution we used was 5.3. (Table 2). It may be seen that all these pigments have specific Rf values. 3) Rf values of vitamins B1 and B2 and folic acid: Vitamins B1 and B2 and folic acid indicate specific Rf values by this procedure. (Table 3). 4) Double spots of lysine picrate and lysine flavinate: Lysine picrate and lysine flavianate show double spots by this method as in Table 4.
- 社団法人 日本農芸化学会の論文