麹菌の生産する抗生物質Oryzacidinに関する研究-4-
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概要
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Since the dilution method for assaying Orizacidin used heretofore took so long days, the need for its new rapid method became apparent. But there are several difficuties such as the test-ing organism. B. hiochi, is anaerobic requiring a special medium, the crystalline pure Oryzacidin is not yet obtained as its working standard, etc. in the selection of assay methods. As the assay method the present paper deals with a cylinder plate technique using B. mesentricus Flügge as the testing organism. The apparatus, procedure and calculation in this technique are just same the official ones of streptomycin, besides 1 mcg of Merzonin (C2H5Hg-S-C6H4COONa, Takeda) per ml is assumed to be 1 unit as its standard and the modified HENNEBEEG's medium is used. According to this technique, the potency of the crude Oryzacidin Na-salt was found to be 10u/mg and that of the tank fermentation broth 30u/ml. The growth of B. hiochi was inhi-bited by 0.033 unit of Oryzacidin.
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