細菌胞子の耐熱性に関する研究-6-

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The spores of P. A. 3679 (Clostridium sporogenes) were suspended and heated in neutral phosphate buffer and then subcultured in liverbroth. Marked increases in the survival times of the spores were observed by the presence of stratified thioglycolate agar on the liver-broth. The survival times of the various spore ncentrations were determined-at the four heating temperatures, 105°, 110°, 115°, and 120°C. The relationships between spore concentrations and survival times of each heating temperature could also be expressed by a general equation, which has been previously obtained on the spores of aerobic bacilli. Thermal-death-time curves for the three different spore concentrations in neutral phosphate buffer were constructed: It was shown that the z values for P. A. 3679 spores are equal to 23°F for every spore concentration, while F values vary with the spore concentration giving the following values. F=12.03 (minutes) for 1 × 106 spores per m l, F=8.69 for 1 × 104 spores per ml., and F=5.79 for 1 × 102 spores per ml. Temperature coefficients of death of P. A. 3679 spores decreased slightly with rising temperature. Consequently, it should be pointed out that the thermal-death-time curve was not essentially a straight line, but was very slightly concaved downward.
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