麹菌の自然変異に関する研究-10-
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概要
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1. Among 16 single-less strains of Asp. sojae tested (4 vitamin-requiring strains, 11 amino acid-requiring strains and 1 base-requring strain), Iysineless (4), histidineless (2), and leucineless (2) mutants strains (Table 1) were comparatively stable. In another strains, however, the reversemutation or segregational mutation were observed. 2. (i) Heterocaryosis has been studies with 8 amino acid deficient mutant strains. In all possible combinations of (lys-+leu-), (lys-+his-), and (his-+bleu-), except certain combinations (3FSR his-+14S leu-) and (3FSR his+17S leu), the prototrophic heterocaryons, able to grow on minimal media, were formed. (ii) Genetic tests for allelism, between 2 strains with similar requirements, have also been carried out. Between 2 leucineless mutants and between 2 histidineless mutants seemed to be allelic, because no positive growth response differing from the controls were obtained, however, among 4 lysineless mutants, both allelic (6st lys-+1st lys-) and non-allelic (6st lys-, 2st lys-, 20S lys-) strains were found. 3. The higher ratios of prototrophic heterocaryon formation were obtained upon those media favoring anastomosis, i.e., lower concentrations of natural media such as. Koji-and Malt-extracts. 4. The segregation ratios of prototrophic heterocaryons were almost constant through 4 generations of pedigree cultures. 5. For the stability of heterocaryon, the lower concentrations of both natural and synthetic media, especially the latter, have been found to be favorable. being more favorable. 6. The phenotype of heterocaryons, as regards to sporulation, color of colony and growthrate etc., have exhibited colonies intermediate of both parents or similar to either parent. Effects resembling hetrocaryotic vigor were also recognized in certain morphological characteristics.
- 社団法人 日本農芸化学会の論文