放線菌変異の遺伝学的研究-1-
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概要
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By means of ultraviolet irradiation, followed by LEDERBERG's replica method, two auxotrophic mutants were induced from one laboratory strain which seemed to belong to the Streptomyees griseoftavus group. One of them, strain No. 261, requires isoleucine and the other strain No. 305, cystine or cysteine. In both cases, the amount of the amino acid required to attain about. half maximal growth was determined to be 10_??_50mg/l. Though the dense inocula (104 of No. 261 or 108 of No. 305, spores per plate, ) provoked the growth of strain even on a minimal medium presumably by a leakage like phenomenon, the colonies appearing on minimal medium were auxotrophic and so far, after repeated subcultures, the strains have been kept stable no reversion being observed.
- 公益社団法人 日本農芸化学会の論文