Competitive ELISA for the Detection of Antibodies to Rift Valley Fever Virus in Goat and Cattle

概要

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Rift Valley fever virus (RVFV) is one of the important emerging viral diseases of serious impact in public health and animal hygiene both in human and animal industry. In this study, we developed monoclonal antibody-based competitive ELISA for the detection of antibodies to RVFV in goat and cattle. The recombinant N protein of RVFV was expressed in E. coli with six-histidine tag and the purified N protein was used for detecting antigen with competitive monoclonal antibody against RVFV antibodies. The competitive ELISA (C-ELISA) could detect antibodies at 9–11 days after inoculation in goat and cattle with the sensitivity of 94.7% (virus neutralization titer >32) and specificity of 99.7%, respectively. In addition, C-ELISA did not show any cross-reactivity with positive sera against Arboviruses such as Akabane, Aino, Chuzan, Ibaraki, and Bovine ephemeral fever virus which are prevalent viral agents in ruminant animals throughout Southeast Asia. The results in present study indicate that C-ELISA is a simple, rapid and convenient sero-diagnostic method for RVFV in goat and cattle.