ガンマー線照射によるトマト雄性不ねん突然変異の誘起ならびに選抜について
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概要
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Four male sterile tomato mutants have been found in the progenies of gamma irradiated plants, two from variety“Shugyoku”and one each from both of“Okitsu No.3”and“Okitsu No.6”. These mutants, except one of Shugyoku, were found in the next generation (M 2) derived from fruits set on lateral branches which regenerated after“internal disbudding”by semilethal gamma exposure. The rate of appearance of ms segregating strains observed, was approximately 3 percent. Breeding results have approved that the male sterility of each mutant is determined by a single recessive gene. No appreciable ovule sterility in any mutant was found.Through the experimental results, the following scheme for the production of ms mutants has been established.1) Preparation of seedlings in a strain promissing as a female parent of F1 variety.2) Irradiating them with ca. 10kR of gamma rays, at an exposure rate of 1-2kR/day, shortly before the blooming of first inflorescence.3) Releasing them until regenerated buds being appeared about a month later from the exposure. One fruit cluster is left on each regenerated branch, and then the seeds are harvested from a fruit in each fruit cluster.4) Raising the plants of next generation as M1 fruit strains. Detecting unfruitful plants and observation on their pollen fertility. Pollination on non pollen plants with use of normal pollen sampled from each of the every fertile plants in the same strain. Test on the fruit set of pollinated flowers, and selection of the normal fruiting plants as ms mutants.5) Raising the next (M3) generation as male parental strains. The ms segregating strains (F1: S1) can be expected in a rate two times higher than that of non segregating strains.6) Investigation on the alternation in any other characters than pollen fertility, especially in crossing ability for F1 hybrid.
- 園芸学会の論文