Experimental and clinical studies on serum amylase activities in pancreatic impairments
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概要
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The amylase activities of the serum, pancreatic juice and organ homogenates of dogs and humans were studied by means of paper electrophoresis, Sephadex gel filtration and polyacrylamide gel electrophoresis and measured by our modification of Noelting & Bernfeld's method.Results obtained were as follows.1) Serum amylase activity of the normal dog; On the paper electrophoresis the major peak appeared at γ-globulin zone of serum protein and the minor peak did at α-β globulin zone.On Sephadex G-100 gel filtration, two peaks were also obtained.2) Serum amylase activity of the dog with pancreatic impairments; When any increase happened, it was mainly at γ-globulin zone.3) Serum amylase activity of the pancreatectomized dog; Remarkable decrease of activity at γ-globulin zone, as well as total activity in serum after the surgery was followed with recovery by degrees before the operation, in a couple of weeks.4) Enzymologic characteristics (optimal pH and Michaelis-Menten's constant) of the separated amylase were different each other, which suggested that isoamylase existed.5) Electrophoresis of various organ homogenates of the dog; Amylase activity was evident only at γ-globulin zone through all the cell fractions of the pancreas by differential centrifugation. The amylase activity of supernatant was dominant at γ-globulin zone and that of small particulate components so at α-β globulin zone, as for the liver and the mucosal cells of intenstine.6) Serum amylase activity of the normal human; Electrophoresis revealed the major peak at γ-globulin zone and the minor peak at albumin zone. There appeared two peaks also in Sephadex G-100 gel filtration.7) Serum amylase activity of cases with acute pancreatitis and chronic pancreatitis; Increase at γ-globulin zone was observed in cases of hyperamylasemia, while there was nothing abnormal in serum amylase pattern in those whose serum amylase activity remained within normal threshold.8) Serum amylase activity of cases with epidemic parotitis; All the cases showed hyperamylasemia, especially increased at γ-globulin zone.9) Comparison of amylase migration among human pancreatic juice, pancreatic homogenate and saliva using polyacrylamide gel plate electrophoresis; Saliva migrated the most, while pancreatic juice and pancreatic homogenate did with no different mobility.
- 財団法人 日本消化器病学会の論文