31P-NMR study on the high energy phosphate metabolism in warm ischemic rat liver caused by hepatic artery and portal vein occlusion.

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An effect on normothermic ischemia on the rat liver metabolism was examined using in vivo 31P-NMR spectroscopy. Energy metabolism was monitored by measuring the ratio of β-ATP/Pi and changes were compared between two groups, untreated rats (Group A) and rats of which spleens were subcutaneously transposed to perform portosystemic collaterals (Group B). In group A, β-ATP/Pi reduced to 0.18 after occlusion of both portal vein and hepatic artery for 10min, and recovered to 0.97 at 120min after reperfusion was initiated. In the case of 30 minischemia, however, it recovered only to 0.53 even at 120min after reperfusion. In contrast to group A, it recovered to 0.81 at 120min after reperfusion following 30min-ischemia in group B. Furthermore, when 10min-ischemia was repeated 3 times with intervals of 10min-perfusion in group B, it recovered to 0.87 as early as 20min after initiation of reperfusion. These results clearly indicate that the prevention of the portal congestion improves recovery from energy metabolic disorder and, in addition, division of total ischemic time with moderate intermission is effective to diminish the metabolic disorder due to occlusion of both hepatic artery and portal vein. However without the prevention of the portal congestion the effect of division of total ischemic time was significantly reduced.
財団法人日本消化器病学会の論文