Changes in cytosolic free Ca++ by histamine stimulation in isolated guinea-pig parietal cells. The second report.
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It was demonstrated in our previous report that cytosolic free Ca++ concentration showed increase following histamine stimulation in the dose dependent manner, by double wave length excitation method using fura-2 as an intracellular Ca++ probe.In this paper, the investiagtion about the source of cytosolic free Ca++ coming out following histamine stimulation was performed. Both Ca++ channel blocker (Verapamil and Diltiazem) and an intracellular Ca++ antagonist (TMB-8) suppressed the increase of cytosolic Ca++ concentration following histamine stimulation. 10-4M varapamil, 10-4M diltiazem and 10-4M TMB-8 suppressed cytosolic Ca++ concentration from 355.9±5.7nM to 213.8±4.9nM, from 330.1±7.3nM to 149.8±1.7nM and from 324.9±6.1nM to 151.3±3.8nM respectively in the values at the point of 20min after 10-4M histamine stimulation.The time course study revealed that the course of increase consisted of two components: the "initial rise" which was observed in the first 1min, followed by the "continuous rise". The initial rise may reflect the Ca++ coming out from the intracellular Ca++ pool and the continuous rise may reflect the Ca++ flowed into cell from outside. Verapamil and diltiazem did not suppress the initial rise, while TMB-8 suppressed it.It is concluded that increase of cytosolic Ca++ concentration following histamine stimulation may be concerned with both extra- and intracellular Ca++ and that at first, intracellular Ca++ may be mobilized.
- 財団法人 日本消化器病学会の論文
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