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We measured macromolecular glycoproteins in the gastric mucosa and studied its location to search for a role of defensive factors in the mechanisms of peptic ulcer formation. The glandular stomach tissues in rats were seperated into three groups; glandular stomach, separated proper mucosal layer and the remaining tissue of the gastric wall. After lyophilizaton, each of the three groups was examined in two ways with and without delipidization respectively. The glycoproteins were extracted with Tris-HCl buffer containing 2% Triton X-100 and a part of the extract was fractionated on Bio-Gel 1.5m column chromatography. The hexoses of all fractions were analyzed by phenol sulphric acid method using galactose as a standard. Macromolecular peak appeared in the proper mucosal layer but disappeared in the other gastric layer after delipidization. The measurement of the quantitative and qualitative changes in the mucus macromolecular glycoproteins has been very troublesome because of difficulties in its procedure. However, this method of applying delipidization made it possible to estimate its contents in the glandular stomach tissues as an index of defensive factor of the stomach. This study will devote to the research of gastric mucus which may play an important role in the mechanisms of gastric ulcer formation.
財団法人日本消化器病学会の論文