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The relationship between the amino acid composition of the culture media or pancreatic hormone and the nitrogen metabolism in primary monolayer cultured adult rat hepatocytes was studied. The composition of the amino acids used in the incubation media was similar to that of the plasma amino acids found in normal human subjects (NAA) as well as in patients with liver cirrhosis (LCAA). Synthesis rates of albumin and total protein were determined from the rates of 14C-leucine incorporation into immunoprecipitable albumin and into trichloroacetic acid-insoluble material. The results obtained were as follow:1) Intact hepatocytes, as well as D-galactosamine-pretreated hepatocytes, when incubated in NAA, synthesized 1.1 to 1.2 times as much protein as hepatocytes incubated in LCAA. In protein synthesis, therefore, NAA was proved to have an advantage over LCAA.2) Intact hepatocytes incubated in the media containing a physiological concentration of insulin (10-9M), glucagon (3×10-11M) and dexamethasone (10-8M) synthesized about 1.5 times as much protein as hepatocytes cultured in the media without these hormones.3) Glucagon at ten times the concentration of the physiological level stimulated protein synthesis in intact hepatocytes, particularly in those hepatocytes cultured in LCAA. The active amino acid transport mechanism may be stimulated by glucagon. These results suggest that hyperglucagonemia in cirrhotic patients may be a physiological response to plasma amino acid imbalance.
- 財団法人 日本消化器病学会の論文