:Part I. Effect of Bile Acid on Endotoxin Masataka IWASAKI
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概要
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Biological properties of endotoxin treated with various bile acids were investigated in this study. The Limulus Amoebocyte Lysate Test (Limulus Test) was performed after mixing the endotoxin solution (0111 B 4, Difco Co., ) with each of the following agents; human bile, direct and indirect bilirubin, and 6 different kinds of bile acids (Deoxycholic acid, Chenodeoxycholic acid, Glycochenodeoxycholic acid, Taurochenodeoxycholic acid, Taurocholic acid, Taurolithocholic acid).The human bile inhibited the Pregel coagulation in Limulus Test, but direct and indirect bilirubin did not inhibit it. One percent solution of each of the six bile acids completely inhibited the Pregel coagulation. Further, Deoxycholic acid, the strongest inhibiting agent, required dilution to 1×10-6% before losing its ability to inhibit the reaction. Our studies revealed similar blood coagulation factors in both the human and Limulus poliphemus.The results of this study suggest that the inhibitory effect of bile acid on the reaction between endotoxin and Pregel is due to the detergent effect of bile acid, possibly by either dissociating endotoxin into nontoxic subunits, or distributing endotoxin into micellar aggregates.
- 財団法人 日本消化器病学会の論文