SEQUENTIAL STUDIES ON EXPERIMENTAL INTESTINAL METAPLASIA AND CARCINOMA IN THE STOMACH OF RATS BY N-PROPYL-N'-NITRO-N-NITROSOGUANIDINE

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Sequential studies on intestinal metaplasia in the glandular stomach of rats induced by N-propyl-N'-nitro-N-nitrosoguanidine(PNNG) were performed. PNNG was given to 56 rats in their drinking water at the level of 100 μg/ml for 44 weeks, and then, tap water until the termination of the experiment. The rats were killed periodically to examine the intestinal metaplasia and tumor in the glandular stomach. The intestinal metaplasia was first found at the 19th week in the glandular stomach of a rat and the incidence of intestinal metaplasia was 57% in the rats killed during the periods between the 19th-28th week. The incidence increased according to the experimental days and 100% of the rats had intestinal metaplasia at the 88th week. Carcinoma in the glandular stomach was first found at the 69th week. There was a great difference in the time of appearance between intestinal metaplasia and gastric carcinoma. In the control rats, intestinal metaplasia was first found at the 50th week and thereafter 20-40% of the rats had intestinal metaplasia. No gastric tumor was fcund in the control rats.Metaplastic glands were observed by transmission electron-microscopy and autoradiography. Metaplastic glands showed irregularly developed microvilli and goblet cells with pale mucin granules by electron-microscopy. Autoradiography showed labeled nuclei at the lower half of the metaplastic glands like doudenal epithelium.
財団法人日本消化器病学会の論文