ENERGY DISPERSIVE X-RAY MICROANALYSIS OF RAT PANCREATIC ACINAR CELLS BY USING FRESH FROZEN THIN SECTIONING METHOD

概要

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Elemental analysis of unfixed and unstained fresh frozen thin sections of rat pancreatic acinar cells was carried out by using an energy dispersive X-ray microanalyser. The freeze substitution method adequately preserved cellular ultrastructure for X-ray microanalysis. The nucleus, rough endoplasmic reticulum, mitochondria with granules, secretory granules and acinar lumen were clearly observed. As a result of X-ray microanalysis of these organelles, the nucleus contained P, K and Cl. The rough endoplasmic reticulum demon-strated P and K. In mitochondrial granules the detection of S, K, P and Cl was seen. And then, the mature secretory granules showed peaks of S, K, P and Cl, however, in immature secretory granules the peak of S was markedly lower. Secretory substances in the acinar lumen demonstrated S, K, P, Si and Cl. As a result of analysis of variable secretory granules, clear differences of the peak of S were concerned between mature secretory granules and immature secretory granules.
財団法人日本消化器病学会の論文