Studies on various anti-tissue antibodies in the liver disease by the immunofluorescence method
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概要
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Anti-tissue antibodies were examined by the immunofluorescence method in the sera of 168 subjects, including 111 patients with various liver diseases, patients with nonhepatic conditions and control subjects.Unfixed 4μ thick cryostat sections of rat stomach, liver and kidney were treated with various dilutions of patient's serum and then stained with fluorescent antihuman IgG.The results were as follows:1) All of the 4 patients with subacute hepatitis showed positive gastric smooth muscle antibody. One was also positive for anti-nuclear antibody in high titers.2) Eight of the 9 patients with primary biliary cirrhosis had positive antibodies to the gastric parietal cell, renal tubular cell and gastric smooth muscle. One showed positive anti-nuclear antibody in high titers.The levels of antibodies expressed by the maximum dilution of serum giving positive staining were relatively high in most patients.In contrast, the patients with extrahepatic biliary obstruction and intrahepatic cholestasis showed a very low incidence of antibodies and when positive, they were very low in titer.Thus, these studies are of great value in the differential diagnosis between primary biliary cirrhosis and extrahepatic biliary obstruction or intrahepatic cholestasis.3) Gastric smooth muscle antibody was positive in 8 of 12 patients with hepatoma. Two of those who were positive also had anti-nuclear antibody in low titers.It was also demonstrated in relatively high incidences in patients with chronic active hepatitis, acute hepatitis and cirrhosis.4) The corticosteroid therapy decreased the dilution titers of these antibodies to an appreciable extent.5) In other liver diseases, anti-tissue antibodies were infrequents, and if present, low in titer.These findings are compatible with the concept that autoimmune reactions involved to a significant extent in the developement and subsequent course of liver disease, especially of chronic liver diseases.
- 財団法人 日本消化器病学会の論文